Journal: bioRxiv

Article Title: Adipose Tissue Inflammation, Oxidative Stress, and Altered Adipogenesis Are Drivers of Dyslipidemia – A Multi-Omics Overview of Dyslipidemia in Obesity

doi: 10.1101/2025.10.15.682716

Figure Lengend Snippet: Correlation plots of leptin with HDL-C and ApoAI with lines of best fit and confidence interval. Spearman’s rank correlation (r), and its Benjamini-Hochberg adjusted p-value ( p ), only significant correlations depicted.

Article Snippet: Plasma adiponectin and leptin concentrations were measured using human Adiponectin/Acrp30 DuoSet ® ELISA (Y1065-05; R&D Systems, Minneapolis, USA) and human Leptin DuoSet ® ELISA (DY398-05; R&D Systems, Minneapolis, USA).

Techniques:

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: Comparison of circulating leptin ( A ), FLI ( B ), sOB-R ( C ) and ghrelin ( D ) pre-surgery and post-surgery (week 1 until 12 months post-surgery). Statistically significant differences were observed in groups post-surgery, * p < 0.01; ** p < 0.001; *** p < 0.005; **** p < 0.0001.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Comparison

Journal: Metabolites

Article Title: Effect of Metabolic and Bariatric Surgery Associated with Changes in Weight Loss, Free Leptin Index, and Soluble Leptin Receptor

doi: 10.3390/metabo15120764

Figure Lengend Snippet: ( A ) Correlation matrix between circulating leptin, ghrelin, FLI, and anthropometric/metabolic variable changes following metabolic and bariatric surgery. Values are repeated-measures correlations (also called intra-individual correlations). Only significant results are shown based on Bonferroni-adjusted p -value. ( B ) Graph of correlations of sOB-R and FLI with leptin.

Article Snippet: Leptin levels were quantified with the Human Leptin DuoSet ELISA (Catalog No. DY398-05, R&D Systems, Inc., Minneapolis, MN, USA) and sOB-R levels with the Human Leptin Receptor DuoSet ELISA (Catalog No. DY389, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: European journal of endocrinology

Article Title: Hypoxia-induced leptin production in human trophoblasts does not protect from apoptosis.

doi: 10.1530/eje.1.01979

Figure Lengend Snippet: Figure 1 Expression profiles of leptin receptor isoforms. JAr cells express both isoforms (long, ObRl variant and short, ObRs var- iant) of the leptin receptor. H2O as template served as a negative control (-).

Article Snippet: Concentrations of leptin in the culture supernatants were measured by using the Human Leptin DuoSet ELISA Development System (R&D Systems) following the recommendations of the manufacturer. b-hCG ELISA Concentrations of b-hCG in the culture supernatants were measured by using the DRG-b-hCG-ELISA kit (DRG Instruments, Marburg, Germany) following the recommendations of the manufacturer.

Techniques: Expressing, Variant Assay, Negative Control

Journal: European journal of endocrinology

Article Title: Hypoxia-induced leptin production in human trophoblasts does not protect from apoptosis.

doi: 10.1530/eje.1.01979

Figure Lengend Snippet: Figure 3 Flow cytometric analysis of apopto- sis in JAr cells. Cells were stimulated for 48 h in the presence of 1 mg/ml leptin (þLeptin) or under hypoxic conditions (1% O2, þ Hypoxia). In addition, hypoxic cells were stimulated using 1 mg/ml leptin (þLeptin þ Hypoxia). Afterwards, annexin V/propidium iodide (PI) staining was used to determine early (annexin Vþ/PI2, R4) and late (annexin Vþ/PIþ, R5) apoptotic cells. In addition, the viable cells (lower left quadrant of the cyto- gram, R2) and necrotic cells (upper right quadrant of the cytogram, R3) were counted.

Article Snippet: Concentrations of leptin in the culture supernatants were measured by using the Human Leptin DuoSet ELISA Development System (R&D Systems) following the recommendations of the manufacturer. b-hCG ELISA Concentrations of b-hCG in the culture supernatants were measured by using the DRG-b-hCG-ELISA kit (DRG Instruments, Marburg, Germany) following the recommendations of the manufacturer.

Techniques: Staining

Journal: European journal of endocrinology

Article Title: Hypoxia-induced leptin production in human trophoblasts does not protect from apoptosis.

doi: 10.1530/eje.1.01979

Figure Lengend Snippet: Figure 4 Cell counts of stimulated JAr cells. Cells were stimulated for 24 h in the presence of 1 mg/ml leptin (Leptin) or under hypoxic conditions (1% O2, Hypoxia). In addition, hypoxic cells were stimulated using 1 mg/ml leptin (L þ H). Afterwards, cells were counted by flow cytometry using beads.

Article Snippet: Concentrations of leptin in the culture supernatants were measured by using the Human Leptin DuoSet ELISA Development System (R&D Systems) following the recommendations of the manufacturer. b-hCG ELISA Concentrations of b-hCG in the culture supernatants were measured by using the DRG-b-hCG-ELISA kit (DRG Instruments, Marburg, Germany) following the recommendations of the manufacturer.

Techniques: Cytometry

Journal: European journal of endocrinology

Article Title: Hypoxia-induced leptin production in human trophoblasts does not protect from apoptosis.

doi: 10.1530/eje.1.01979

Figure Lengend Snippet: Figure 5 Equal expression of caspase-3 and caspase-9 in JAr cells after exposure to hypoxia in the presence or absence of lep- tin. Cells were stimulated for 48 h in the presence of 1 mg/ml leptin (L) or under hypoxic conditions (1% O2, H) or with a combination of both stimuli (H/L). Afterwards, cells were lysed and subjected to Western blot analysis for the expression of caspase-3 (Casp 3) and -9 (Casp 9). Lysates of untreated cells were used as controls (C).

Article Snippet: Concentrations of leptin in the culture supernatants were measured by using the Human Leptin DuoSet ELISA Development System (R&D Systems) following the recommendations of the manufacturer. b-hCG ELISA Concentrations of b-hCG in the culture supernatants were measured by using the DRG-b-hCG-ELISA kit (DRG Instruments, Marburg, Germany) following the recommendations of the manufacturer.

Techniques: Expressing, Western Blot